Figure 4.

In growth phase Dictyostelium cells migrating in microchannels along a gradient of folate, the centrosome is preferentially located behind the nucleus. (a) Scheme (left) and representative microscopy images (right) of a Dictyostelium cell migrating in a microchannel along a folate gradient. The cell shape is highlighted by a yellow dashed line. Images were taken with 10 s-frame intervals. Time is indicated in min and s at the top. (b) Quantification of the centrosome position during migration in microchannels along a gradient of folate. N = 6, number of cells = 40. (c) Quantification of the distance between nucleus and centrosome of Dictyostelium cells migrating in microchannels in a folate gradient (n = 6, cells = 40). (d) Displacement of tracks for nucleus (red) and centrosome (green) recorded for one cell over time. (e) Migration speed in microchannels determined for growth phase Dictyostelium cells moving along a gradient of folate and for aggregation competent cells moving in a gradient of cAMP. * p < 0.05, ** p < 0.01, **** p < 0.0001 are significant, and ns = not significant.