Figure 5.

Identification of proliferating neuroblasts, chromaffin cells, and NESTIN-expressing cells. Proliferating cells were identified by a short 4 h EdU pulse, followed by immunostaining for PHOX2B, TH, or NESTIN combined with EdU-staining using the Click-iT protocol. Cultures of sympathetic neuroblasts (A,B), chromaffin cells (C,D), and Nestin-expressing cells (NECs) (E) are shown. For quantification PHOX2B/EdU rather than TH/EdU staining was used since nuclear staining resulted in better cell identification. Magnification bar, 60 μm.