Figure 8.

Trans-differentiation of chromaffin cells in culture. Double-immunostaining shows that while TH (A) and TUBB3 (B) were maintained during the 6d culture period, the chromaffin cell-specific proteins PNMT, DLK1, and SGII are lost or decreased (E,F,G). Increased ISLET1 expression does not reach significance (C,H). PERIPHERIN is expressed by a very low number of cells both at 3d and 6d in culture (D,H). The results are quantified in (H). Significant differences marked by * and *** indicate p-values < 0.05 and <0.001, respectively. Magnification bar, 60 μm.