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. 2022 May 27;11(11):1760. doi: 10.3390/cells11111760

Figure 1.

Figure 1

Progesterone attenuates estrogen-responsive genes. (a) RT-PCR for IHH or PGR from RNA after treatment of organoid isolated from 3 different donors with vehicle (V) estrogen (E2) or estrogen with medroxyprogesterone acetate (MPA+E2). *, **, **** p < 0.05, 0.01, and 0.0001, respectively, after one way ANOVA with uncorrected Fisher’s LSD test, n = 3 samples per group. (b) RT-PCR for IHH or PGR from RNA after treatment of organoid isolated from donor 2 with V, E2, MPA+E2, or RU486+MPA+E2. **, **** p < 0.01 and 0.0001, respectively after one way ANOVA with uncorrected Fisher’s LSD test, n = 3 samples per group. (c) Hierarchical cluster heatmap of E2 vs. V (E2) or E2+MPA vs. V (E2+MPA) differentially expressed genes (2-fold, FDR p-value < 0.05) of RNA from 2 different donors.