Table 1.
The sequences of primers used in this study.
| Purpose | Gene | Sequence (5′—3′) | Size (bp) | GenBank Accession Number |
|---|---|---|---|---|
| qRT-PCR for mRNA expression |
MYF5 | F: AGACGCCTCAAGAAGGTCAA | NM_001278775 | |
| R: TCCTGCAGGCTCTCAATGTA | 128 | |||
| MYOD1 | F: TGCAAACGCAAGACCACTAA | NM_001002824 | ||
| R: GCTGATTCGGGTTGCTAGAC | 127 | |||
| MYOG | F: CAGTGAATGCAGTTCCCACA | NM_001012406 | ||
| R: GGTGAGGGAGTGCAGATTGT | 130 | |||
| MEF2A | F: TGATGCGGAATCATAAAATCG | NM_001099698 | ||
| R: GCACCAGTAGTTCCAACCAAA | 358 | |||
| MEF2C | F: CGAGATACCCACAACACACG | NM_001044540 | ||
| R: CGCTTGACTGAGGGACTTTC | 175 | |||
| MEF2D | F: TCACTGCAGTTCAGCAATCC | XM_021089672 | ||
| R: AGGCCAGGAGACACACTGTT | 128 | |||
| HDAC1 | F: GGAAATCTATCGCCCTCACA | XM_013999116 | ||
| R: AAACACCGGACAGTCCTCAC | 157 | |||
| HDAC3 | F: CAACCAGGTGGTGGACTTCT | NM_001243827 | ||
| R: GCAGAGGGATGTTGAAGCTC | 152 | |||
| HDAC5 | F: AGATGCACTCCTCCAGTGCT | XM_021066892 | ||
| R: GGATGATGGCAAATCCATTC | 102 | |||
| HDAC6 | F: ATGGACGGGTATTGCATGTT | XM_003360315 | ||
| R: GCGGTGGATGGAGAAATAGA | 168 | |||
| HDAC7 | F: CGTCCCCTACAGAACTCTCG | XM_021092604 | ||
| R: TCAGGTTGGGCTCAGAGACT | 146 | |||
| HDAC8 | F: GGTGACGTGTCTGATGTTGG | XM_021080459 | ||
| R: AGCTCCCAGCTGTAAGACCA | 165 | |||
| HDAC9 | F: AACTGAAGCAACCAGGCAGT | XM_021102482 | ||
| R: CCCAACTTGTCCCAGTGAGT | 149 | |||
| HDAC10 | F: TCCATCCGAGTACCTTCCAC | XM_021091335 | ||
| R: GGCTGCTATGGCCACACTAT | 179 | |||
| HDAC11 | F: GACAAGCGCGTGTACATCAT | XM_021069384 | ||
| R: AGGTTCCTCTCCACCTTCGT | 143 | |||
| TGFB1 | F: CGTGCTAATGGTGGAAAGCG | XM_021093503 | ||
| R: AGAGCAATACAGGTTCCGGC | 122 | |||
| SMAD2 | F: GCAATCTTTGTGCAGAGCCC | NM_001256148 | ||
| R: ACACGGCTTCAAAACCCTGA | 157 | |||
| SMAD3 | F: GCTGGACGACTACAGCCATT | NM_214137 | ||
| R: TGTGGTTCATCTGGTGGTCG | 140 | |||
| SMAD7 | F: CCAACTGCAGACTGTCCAGA | XM_005659454 | ||
| R: CAGGCTCCAGAAGAAGTTGG | 106 | |||
| STAT5A | F: GAGGTGCTGAAGAAGCATCA | NM_214290 | ||
| R: GGCTTCAGATTCCACAGGTT | 200 | |||
| SP1 | F: TGCAGCAGAATTGAGTCACC | XM_005652567 | ||
| R: ACTGCTGCCACTTTGTTCCT | 180 | |||
| CEBPB | F: GCTTGAACAAGTTCCG | NM_001199889 | ||
| R: CAAGAAGACCGTGGATAAGC | 209 | |||
| Nrf2 | F: GTGCCTATAAGTCCCGGTCA | XM_013984303 | ||
| R: ATGCAGAGCTTTTGCCCTTA | 108 | |||
| DNMT1 | F: GCGGGACCTACCAAACAT | NM_001032355 | ||
| R: TTCCACGCAGGAGCAGAC | 133 | |||
| DNMT3a | F: CTGAGAAGCCCAAGGTCAAG | NM_001097437 | ||
| R: CAGCAGATGGTGCAGTAGGA | 238 | |||
| HPRT1 | F: AACCTTGCTTTCCTTGGTCA | NM_001032376 | ||
| R: TCAAGGGCATAGCCTACCAC | 150 | |||
| qRT-PCR for miRNA expression |
ssc-miR-15a | F: TAGCAGCACATAATGGTTTGT | - | MIMAT0007753 |
| ssc-miR-15b | F: TAGCAGCACATCATGGTTTACA | - | MIMAT0002125 | |
| ssc-miR-17-5p | F: CAAAGTGCTTACAGTGCAGGTAG | - | MIMAT0007755 | |
| ssc-miR-20a-5p | F: TAAAGTGCTTATAGTGCAGGTA | - | MIMAT0002129 | |
| ssc-miR-21-5p | F: TAGCTTATCAGACTGATGTTGA | - | MIMAT0002165 | |
| ssc-miR-92a | F: TATTGCACTTGTCCCGGCCTGT | - | MIMAT0013908 | |
| ssc-miR-106a | F: AAAAGTGCTTACAGTGCAGGTAGC | - | MIMAT0002118 | |
| Bisulfite sequencing PCR | SMAD7-Bis | F: TGATTTTTAAGTATTTTGAAAGTTG | ||
| R: TCTCTAAAATACATTTAACTAACTAAC | 245 | |||
| ChIP-qPCR | Region I | F: TATGCCTCATGCACAGCACC | ||
| R: CCCATGCACAGGGAAAGACA | 93 | |||
| Region II | F: ATTGCAGCCTCTGTGGCTTA | |||
| R: GACCTAGGGATGCCAAGCAG | 91 | |||
| Region III | F: TGGTCCTTTGCCCTACCAAC | |||
| R: ATCCCCTTAGCCTGCGTTTT | 89 | |||
| Region IV | F: ACTCTCTGACTCTTAAGCACCT | |||
| R: AGGTTACCTTCCTAGCAGACCT | 106 | |||
| Region V | F: CGGTCCAGTCCGGTGTAAAT | |||
| R: CGTTTTGCCTTAAAGGCCCTG | 105 |