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. 2022 May 27;11(11):1765. doi: 10.3390/cells11111765

Figure 5.

Figure 5

Drought response of peroxisome biogenesis and autophagy markers. (A–C) Transcription level of peroxisome fission genes (A), peroxisome fission gene PEX11-C homoeologs from chromosome 7A (B) and autophagy flux marker ATG (C). qRT-PCR transcription levels were normalized to housekeeping gene RNase L inhibitor-like protein. Values above the dashed line indicate up-regulation; values below the line indicate down-regulation. (D) Western blotting with ATG8 antibody or following immunodepletion of the antibody with the ATG8 protein. Pre-incubation of the antibody with the antigen abrogates recognition of ATG8 in leaf total protein extract. (E) Western blotting with anti-ATG8 of total protein extracts from leaves of control and drought-stressed Drysdale and Hollis plants. Bars and numbers indicate the position and corresponding size of molecular weight markers. (F) Colloidal Silver staining of the corresponding Western blotting membrane showing total protein. Bars and numbers indicate the position and corresponding size of molecular weight markers. (G) Quantification of ATG8 protein abundance on the Western blotting membranes. p-values represent Student t-test results of three technical replicates of extracts from three biological replicates (individual plants). (H) The ratio of ATG8 protein in extracts from drought-stressed leaves to that in control leaves. p-values represent Student t-test results of three technical replicates of extracts from three biological replicates (individual plants).