Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 24;14(11):2580. doi: 10.3390/cancers14112580

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

MUC1-C induces expression of U-ISGF3 target genes. (A) BT-549/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for STAT2 and IRF9 mRNA levels by qRT-PCR. The results (mean ± SD of 4 determinations) are expressed as relative mRNA levels compared to that obtained for vehicle-treated cells (assigned a value of 1). (B) Lysates from (i) BT-549/tet-MUC1shRNA cells treated with vehicle of DOX for 7 days and (ii) BT-549/CshRNA and BT-549/MUC1shRNA#2 cells (right) were immunoblotted with antibodies against the indicated proteins. (C). RNA-seq performed in triplicate on BT-549/tet-MUC1shRNA treated with vehicle (gray bars) or DOX (red bars) for 7 days was analyzed for expression of the indicated U-ISGF3 target genes. The results are expressed as the mean ± SD of 3 determinations. IRDS genes are highlighted with red boxes. (D) BT-549/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for the indicated IRDS mRNA levels by qRT-PCR. The results (mean ± SD of 3 determinations) are expressed as relative mRNA levels compared to that obtained for vehicle-treated cells (assigned a value of 1). (E,F) Scatter plots showing correlations of MUC1 with the indicated IRDS genes in TNBCs from the TCGA-BRCA (E) and METABRIC (F) cohorts. (G) Scatter plots of the correlation between MUC1 and the indicated IRDS genes in single TNBC cells as analyzed from the GSE118389 scRNA-seq dataset. The asterisk (*) denotes a p-value < 0.05. Uncropped Western Blots can be found at supplemental original images.