Figure 1.

Gallic acid induces cell cycle arrest and nucleolus disintegration. (A) Dose response curve of GA for non-tumoral CRL1790, tumoral SW480 and metastatic SW620 cells after treatment during 48 h. (B) Chemical structure of GA selected as antitumoral candidate and natural sources where it can be found. (C) Histograms of SW480 cells treated with DMSO (non-treated, NT) or treated with GA IC₅₀ for 24 h in propidium iodide flow cytometry analysis. (D) Immunofluorescence images of SW480 cells treated with vehicle DMSO (NT) or treated with GA IC₅₀ for 6 h, and stained for nucleolin (NCL), fibrillarin (FBL) or POLR1A. Merged images with DAPI for DNA counterstaining are also shown. Scale bar, 10 µm.