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. Author manuscript; available in PMC: 2022 Jun 9.
Published in final edited form as: J Med Chem. 2021 Jun 4;64(12):8755–8774. doi: 10.1021/acs.jmedchem.1c00758

Table 2.

Antiviral activity of potent leads against different serotypes of EV-D68 and EV-A71 in RD cells

EV-strains graphic file with name nihms-1810554-t0048.jpg graphic file with name nihms-1810554-t0049.jpg graphic file with name nihms-1810554-t0050.jpg graphic file with name nihms-1810554-t0051.jpg
EV-D68 US/KY/14-18953 (Clade D) EC50 = 0.1 ± 0.03
CC50 = 200.0 ± 10.8
SI50 = 2000		 EC50 = 0.2 ± 0.1
CC50 = 236.3 ± 20.5
SI50 = 1183		 EC50 = 0.03 ± 0.001
CC50 = 160.9 ± 12.8
SI50 = 5363.3		 EC50 = 1.0 ± 0.1
CC50 = 116.6 ± 16.1
SI50 = 116.6
US/MO/14-18947 (Clade B1) EC50 = 0.1 ± 0.03
CC50 = 200.0 ± 10.8
SI50 = 2000		 EC50 = 0.2 ± 0.05
CC50 = 236.3 ± 20.5
SI50 = 1183		 EC50 = 0.07 ± 0.01
CC50 = 160.9 ± 12.8
SI50 = 2298.6		 EC50 = 0.4 ± 0.2
CC50 = 116.6 ± 16.1
SI50 = 291.5
US/MO/14-18949 (Clade B1) EC50 = 0.1 ± 0.02
CC50 = 200.0 ± 10.8
SI50 = 2000		 EC50 = 0.2 ± 0.1
CC50 = 236.3 ± 20.5
SI50 = 1183		 EC50 = 0.08 ± 0.01
CC50 = 160.9 ± 12.8
SI50 = 2011.3		 EC50 = 0.7 ± 0.4
CC50 = 116.6 ± 16.1
SI50 = 166.6
US/IL/14-18956 (Clade B2) EC50 = 0.04 ± 0.01
CC50 = 200.0 ± 10.8
SI50 = 5000		 EC50 = 0.2 ± 0.04
CC50 = 236.3 ± 20.5
SI50 = 1183		 EC50 = 0.07 ± 0.01
CC50 = 160.9 ± 12.8
SI50 = 2298.6		 EC50 = 0.4 ± 0.1
CC50 = 116.6 ± 16.1
SI50 = 291.5
US/IL/14-18952 (Clade B2) EC50 = 0.04 ± 0.01
CC50 = 200.0 ± 10.8
SI50 = 5000		 EC50 = 0.2 ± 0.03
CC50 = 236.3 ± 20.5
SI50 = 1183		 EC50 = 0.09 ± 0.01
CC50 = 160.9 ± 12.8
SI50 = 1787.8		 EC50 = 0.7 ± 0.02
CC50 = 116.6 ± 16.1
SI50 = 166.6
EV-A71 Tainan/4643/1998 EC50 = 0.1 ± 0.04
CC50 = 200.0 ± 10.8
SI50 = 2000		 EC50 = 0.2 ± 0.03
CC50 = 236.3 ± 20.5
SI50 = 1183		 EC50 = 0.2 ± 0.04
CC50 = 160.9 ± 12.8
SI50 = 804.5		 EC50 = 7.4 ± 1.7
CC50 = 236.3 ± 20.5
SI50 = 31.9
USA/CT/2016-19519 EC50 = 0.06 ± 0.01
CC50 = 200.0 ± 10.8
SI50 = 3333.3		 EC50 = 0.1 ± 0.04
CC50 = 236.3 ± 20.5
SI50 = 2363		 EC50 = 0.06 ± 0.01
CC50 = 160.9 ± 12.8
SI50 = 2681.7		 EC50 = 1.3 ± 0.4
CC50 = 236.3 ± 20.5
SI50 = 181.8
A71 MP4 EC50 = 0.1 ± 0.03
CC50 = 200.0 ± 10.8
SI50 = 2000		 EC50 = 0.3 ± 0.02
CC50 = 236.3 ± 20.5
SI50 = 787.7		 EC50 = 0.2 ± 0.04
CC50 = 160.9 ± 12.8
SI50 = 804.5		 EC50 = 2.0 ± 0.3
CC50 = 236.3 ± 20.5
SI50 = 118.2
a

Antiviral efficacy EC50 was determined using the CPE assay in RD cells.

b

Cytotoxicity CC50 was determined using the neutral red uptake method.

c

N.T.= not tested. The results are the mean ± standard deviation of three repeats. SI50 = selectivity index (CC50/EC50).