Figure 5.

Acute blockade of CD200R1 does not affect myelin clearance after crush injury. Representative images of Luxol Fast Blue (LFB) staining in naive nerve and after crush injury at seven and 28 dpi. Scale bar: 50 µm (A). Graph shows LFB staining represented as a percentage of area stained (B). Representative images of Oil Red O (ORO) lipid droplet accumulation at seven days post-crush in animals treated with vehicle and anti-CD200R (Scale bar: 50 µm) (C) and quantification of the area stained with Oil Red O represented as a percentage of area stained (D). Quantification of the total number of macrophages at seven days post-injury (E) evaluated by FACS analysis and the percentage of macrophages expressing markers of activation as Ly6C, CD16/32 and CD206 (F).