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. 2000 Jan;66(1):170–175. doi: 10.1128/aem.66.1.170-175.2000

TABLE 2.

Effect of SOD and diaphorase on extent of semiquinone autoxidationa

Amt of semiquinone produced (nmol) % Autoxidation of:
MBQ·−
DBQ·−
Control With SOD With diaphorase Control With SOD With diaphorase
50 5.2 43.6 28.0 45.1
100 4.5 30.6 13.8 19.6 40.0 39.9
200 3.5 24.0 14.7 14.0 37.0 41.7
300 2.3 18.6 12.1 37.2
400 2.0 15.4 13.1 10.5 34.6 40.5
500 1.7 12.4 9.6 33.9
a

The extent of semiquinone autoxidation was calculated from H2O2 levels shown in Fig. 2 (control and SOD columns) and Fig. 3 (diaphorase column), taking into account the stoichiometry of the O2·− dismutation reaction and the amount of semiquinone produced by laccase during the entire reactions.