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. 2022 Jun 2;11(11):1817. doi: 10.3390/cells11111817

Figure 1.

Figure 1

Experimental design of the chronic subordinate colony housing (CSC, 19 days) paradigm and effects of CSC exposure and i.c.v. drug-treatment on body weight. (A) Schematic illustration of the CSC paradigm. (B) Chemical structure of the used mGlu7-selective orthosteric-like antagonist XAP044. During 19 days of CSC, (C) body weight gain (day 20–day 1) (factor housing: F1,147 = 0.197, p = 0.658; factor treatment: F3,147 = 2.102, p = 0.102; housing*treatment interaction: F3,147 = 0.718, p = 0.543) and (D) body weight development (factor time: F6,528 = 104.692, p ≤ 0.001; factor treatment: F3,88 = 1.176, p = 0.323; time*treatment interaction: F18,528 = 5.979, p ≤ 0.001) were assessed in SHC and CSC mice treated either with VEH (5% DMSO) or XAP044 at different doses. White bar, SHC; black bar, CSC. n = 7–35 per treatment and housing group. Data represent the mean ± SEM. * p ≤ 0.05, ** p ≤ 0.01 VEH-CSC vs. VEH-SHC in (D); + p ≤ 0.05 XAP(100)-CSC vs. XAP(100)-SHC in (D); # p ≤ 0.05, ## p ≤ 0.01 XAP(100)-CSC vs. VEH-CSC in (C,D); two-way ANOVA followed by Bonferroni post hoc analysis or independent Student’s t-test (C) or repeated measures ANOVA, followed by Bonferroni post hoc analysis or independent Student’s t-test (D).