Table 1.
Antibody | Brand | Catalogue | Dilution |
---|---|---|---|
SPARC | Cell Signaling | 5420 | 1:1000 |
ZEB1 | eBioscience | 14974182 | 1:1000 |
E-cadherin | BD Transduction Laboratories | 610181 | 1:1000 |
Vimentin | AbCam | Ab8978 | 1:2000 |
Integrin β1 | Cell Signaling | 9669T | 1:1000 |
Integrin β3 | Cell Signaling | 13166T | 1:1000 |
Integrin β4 | Cell Signaling | 14803T | 1:1000 |
Integrin β5 | Cell Signaling | 3629T | 1:1000 |
Integrin α5 | Cell Signaling | 4705T | 1:1000 |
Integrin αv | Cell Signaling | 4711T | 1:1000 |
FAK | Cell Signaling | 13009T | 1:1000 |
P-FAK (Y397) | Cell Signaling | 8556T | 1:1000 |
P-FAK (Y576/577) | Cell Signaling | 3281T | 1:1000 |
P-FAK (Y925) | Cell Signaling | 3284T | 1:1000 |
Actin | MP Biomedicals | 691002 | 1:5000 |
Goat anti-Rabbit IgG HRP | Jackson Immunoresearch | 111-035-003 | 1:10,000 |
Goat anti-Mouse IgG HRP | Jackson Immunoresearch | 115-035-003 | 1:10,000 |
Abbreviations: SPARC—secreted protein acidic and rich in cysteine; ZEB1—zinc finger E-box-binding homeobox 1; FAK—focal adhesion kinase; HRP—horseradish peroxidase.