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. 2022 May 24;23(11):5874. doi: 10.3390/ijms23115874

Table 1.

Antibodies used for Western blot.

Antibody Brand Catalogue Dilution
SPARC Cell Signaling 5420 1:1000
ZEB1 eBioscience 14974182 1:1000
E-cadherin BD Transduction Laboratories 610181 1:1000
Vimentin AbCam Ab8978 1:2000
Integrin β1 Cell Signaling 9669T 1:1000
Integrin β3 Cell Signaling 13166T 1:1000
Integrin β4 Cell Signaling 14803T 1:1000
Integrin β5 Cell Signaling 3629T 1:1000
Integrin α5 Cell Signaling 4705T 1:1000
Integrin αv Cell Signaling 4711T 1:1000
FAK Cell Signaling 13009T 1:1000
P-FAK (Y397) Cell Signaling 8556T 1:1000
P-FAK (Y576/577) Cell Signaling 3281T 1:1000
P-FAK (Y925) Cell Signaling 3284T 1:1000
Actin MP Biomedicals 691002 1:5000
Goat anti-Rabbit IgG HRP Jackson Immunoresearch 111-035-003 1:10,000
Goat anti-Mouse IgG HRP Jackson Immunoresearch 115-035-003 1:10,000

Abbreviations: SPARC—secreted protein acidic and rich in cysteine; ZEB1—zinc finger E-box-binding homeobox 1; FAK—focal adhesion kinase; HRP—horseradish peroxidase.