Figure 3.

Metabolic switch modulates tissue-specific neuro-modulator transcripts expression. (a–c) Transcriptional expression profiling of Insulin-like-peptides, neuropeptides, neurohormones, and receptor genes in the brain tissue during the metabolic switch. Statistical analysis using two-way ANOVA and Tukey’s test implied that the expression change of the respective genes is statistically significant for insulin-like-peptides p ≤ 0.007; neuropeptides and receptors p ≤ 0.009, but for neuro-hormones, it was non-significant p ≤ 0.2 (n = 25, N = 4); (d) Relative expression profiling of a subset of neuromodulator genes in the midgut of naïve and blood-fed mosquitoes at the same time point described above. Statistical analysis using two-way ANOVA implied that the expression change of the respective genes is statistically significant p ≤ 0.005 (n = 12, N = 4); (e) Transcriptional profiling of genes involved in signal transduction during vitellogenesis in the ovary. Statistical analysis using two-way ANOVA and Tukey’s test indicated that the expression change of the respective genes was statistically significant at p ≤ 0.002 (n = 12, N = 4); (f) Relative gene expression analysis of diuresis-related genes in the Malpighian tubule of naïve and blood-fed mosquitoes. Statistical analysis using two-way ANOVA and Tukey’s test indicates that the expression change of the respective genes is non-significant at p ≤ 0.4 (n = 25, N = 4). For Statistical analysis, all the time points and all the transcripts are compared together using two-way ANOVA and the means of the time points showed statistically significant. (n = number of mosquitoes from which the respective tissue was dissected and pooled for each independent experiment; N = number of biological replicates).