Table 3.
Primer pairs and target genes used for the developed mPCR assay.
| Primer | Primer Sequence (5′–3′) | Target Gene | Target | Product Size (bp) | Reference |
|---|---|---|---|---|---|
| stx1-F stx1-R |
TGTAACTGGAAAGGTGGAGTATACA GCTATTCTGAGTCAACGAAAAATAAC |
stx1 | STEC | 210 | [19] |
| stx2-F stx2-R |
ATCAGTCGTCACTCACTGGT CTGCTGTCACAGTGACAAA |
stx2 | STEC | 110 | [20] |
| eae-F eae-R |
TCAATGCAGTTCCGTTATCAGTT GTAAAGTCCGTTACCCCAACCTG |
eae | STEC | 482 | [21] |
| hlyA-F hlyA-R |
AAATCATCGACGGCAACCT GGACGATGTGAAATGAGC |
hlyA | L. monocytogenes | 348 | [22] |
| invA-F invA-R |
GTGAAATTATCGCCACGTTCGGGCAA TCATCGCACCGTCAAAGGAACC |
invA | Salmonella spp. | 284 | [23] |
| IAC-F IAC-R |
CAGGATTGACAGAGCGAGGTATG CGTAGTTAGGCCACCACTTCAAG |
ori | pUC19 | 65 | [24] |
stx1 and stx2 gene: Shiga toxin gene; eae gene: Intimin gene; invA gene: Invasion gene; hlyA gene: Hemolysin A gene.