TABLE 2.
Chemical shift and assignment of resonances for 13C-NMR spectrum of recombinant strain ZM4(pZB5) culture supernatant from xylose (50 g/liter) fermentationa
| Xylose or product | Resonance at carbon:
|
||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| Xylose α | 93.1 | 72.5 | 73.9 | 70.4 | 61.9 |
| Xylose β | 97.5 | 75.1 | 76.8 | 70.2 | 66.1 |
| Xylitol | 63.9 | 73.2 | 72.0 | 73.2 | 63.9 |
| Ethanol | 58.3 | 17.8 | |||
| Glycerol | 64.0 | 73.5 | 64.0 | ||
| Acetate | 182.1 | 24.1 | |||
| Lactate | 183.2 | 69.3 | 20.9 | ||
| Acetoin | 216.2 | 73.9 | 25.8 | 19.1 | |
| Dihydroxyacetone | 66.0 | 213.1 | 66.0 | ||
| Dihydroxyacetone hydrate | 64.9 | 96.5 | 64.9 | ||
a
Chemical shifts expressed as parts per million downfield from TMS. DMSO was used as the internal standard at 39.5 ppm. NMR operation: 90° pulse, 2-s pulse repetition, 16,000 scans accumulated.