TABLE 1.
Recovery of viruses added to different aqueous solutions by adsorption to cellulose nitrate membrane filters and a plaque assay performed with a monolayer
| Type of sample | Pore size of the membrane filter (μm) | Treatment of the sample | Vol filtered (ml) | No. of tests | % Recoverya |
|---|---|---|---|---|---|
| Tap water | 3 | pH 3.5 | 500 | 10 | 157.0 (34.5) |
| Tap water | 3 | 0.05 M MgCl2 | 500 | 10 | 151.0 (24.1) |
| Seawater | 3 | pH 3.5 | 500 | 16 | 153.0 (96.5) |
| Seawater | 3 | pH 3.5 | 100 | 6 | 179.0 (94.0) |
| Seawater | 5 | pH 3.5 | 500 | 6 | 154.0 (81.0) |
| Seawater | 5 | pH 3.5 | 100 | 6 | 176.0 (86.0) |
| 0.25 M glycine | 3 | pH 3.5 | 500 | 10 | 51.0 (17.8) |
| 0.25 M glycine | 3 | 0.05 M MgCl2 | 500 | 16 | 73.3 (15.3) |
| 0.25 M glycine | 3 | 0.05 M MgCl2 | 100 | 10 | 166.0 (69.0) |
a
Percentage of viruses recovered after filtration compared to the number of viruses determined by the standard plaque assay method. The values are means (standard deviations).