Skip to main content
. 2022 May 25;23(11):5955. doi: 10.3390/ijms23115955

Figure 6.

Figure 6

Morphological and morphometric analysis of C2C12 myotubes. C2C12 myoblasts were cultured in DM for 7 days. Once differentiated in myotubes, cells were incubated for 48 h with DM (A, control); with 1 μM DEX in the absence (B) or presence of 30 μM glucoraphanin (C), 150 μM L-cysteine (D), and 150 μM mercaptopyruvate (E). Cells were fixed and stained with FITC-conjugated phalloidin to highlight F-actin organization (green) and with DAPI, for nuclei staining (blue). Scale bar (white line): 50 μm. Morphometric analyses were carried out to provide: the length in μm of the myotube diameter (F); the multinucleated cells percentage (G) (percentage ratio of the number of multinucleated cells and the number of total cells, considered as 100%); and the number of nuclei in each myotube (H). The analyses were conducted in at least 3 random fields of each experimental group. Values are expressed as the mean ± S.E.M. of 3 different experiments. * p < 0.05 versus control; ^ p < 0.05 versus DEX.