Figure 1.

High-fat-diet-fed mice develop NASH: (A) HE stain of liver slices from animals fed either the control (ND) or the high fat diet (HFD); where indicated, animals received MSC (+MSC) or were left untreated (−MSC). Arrows indicate inflammatory foci. Pictures are representative for 2 different animals out of each group. (B) Determination of NASH scores using either the NAS [34] or the SAF [35] score; 3 (ND − MSC), 4 (ND + MSC), 6 (HFD − MSC) and 8 (HFD + MSC) animals per group are included for analysis. Numbers in columns represent the score according to the respective scoring system used (cf. Materials and Methods). The pathological evaluation of the scores revealed either no NAFLD (green), NASH (orange), or borderline and NAFLD (yellow).