Table 1.
Antibodies used for immunofluorescence.
| Antibodies | Host | Dilution | Source | |
|---|---|---|---|---|
| Primary | Anti-Cx37/GJA4 ab181701 | Rabbit | 1:500 | Abcam (Cambridge, UK) |
| Anti-Cx40/GJA5 ab213688 | Rabbit | 1:100 | Abcam (Cambridge, UK) | |
| Anti-Cx43&GJA1 ab87645 | Goat | 1:200 | Abcam (Cambridge, UK) | |
| Anti-Cx45/GJA7 ab135474 Anti-pannexin 1/PANX1 |
Rabbit Rabbit |
1:100 1:300 |
Abcam (Cambridge, UK) Merck KGaA (Darmstadt, Germany) |
|
| Secondary | Anti-Goat IgG, Alexa Fluor® 488, ab150129 | Donkey | 1:400 | Abcam (Cambridge, UK) |
| Anti-Rabbit IgG, Alexa Fluor® 488, 711-545-152 | Donkey | 1:400 | Jackson Immuno Research Laboratories, Inc. (Baltimore, PA, USA) | |
Data Acquisition, Semi-Quantitative and Statistical Analysis.