Table I.
List of antibodies used for immunophenotyping.
| Antigen | Fluorochrome | Clone | Company | Isotype |
|---|---|---|---|---|
| CD14 | BUV395 | MφP9 | BD Biosciences Cat#563561 | Mouse BALB/c IgG2b, κ |
| CD16 | APC-H7 | 3G8 | BD Biosciences Cat#560195 | Mouse CDF1 IgG1, κ |
| CCR5 (CD195) | BV711 | 2D7/CCR5 | BD Biosciences Cat#563395 | Mouse C57BL/6 IgG2a, κ |
| CD181 (CXCR1) | PE-Cy5 | 5A12 | BD Biosciences Cat#551081 | Mouse IgG2b, κ |
| CD182 (CXCR2) | APC | 6C6 | BD Biosciences Cat#551127 | Mouse IgG1, λ |
| CD142 (Tissue Factor) | BUV737 | HTF-1 | BD Biosciences Cat#748835 | Mouse IgG1, κ |
| CD162 (PSGL) | BV786 | KPL-1 | BD Biosciences Cat#743483 | Mouse BALB/c IgG1, κ |
| CCR2 | BV650 | LS132.1D9 | BD Biosciences Cat#747849 | Mouse BALB/c IgG2a, κ |
| IL-6 | PE-CF594 | MQ2–13A5 | BD Biosciences Cat#563543 | Rat IgG1 |
| IL-8 | BV421 | G265–8 | BD Biosciences Cat#563310 | Mouse IgG2b |
| TNF | BV605 | MAb11 | Biolegend Cat#502936 | Mouse IgG1, κ |
| CXCL10 | Alexa Fluor 488 | 4NY8UN | Invitrogen Cat#53–9744-42 | Mouse / IgG2b, κ |
| IL-1RA | PE | AS17 | BD Biosciences Cat#340525 | Mouse IgG1 |
| CD3 | PerCP-Cy5.5 | UCHT1 | BD Biosciences Cat#560835 | Mouse BALB/c IgG1, κ |
| CD4 | BUV737 | SK3 | BD Biosciences Cat# 612748 | Mouse BALB/c IgG1, κ |
| CD8 | BUV395 | RPA-T8 | BD Biosciences Cat#563795 | Mouse IgG1, κ |
| CD25 | PE-Cy7 | M-A251 | BD Biosciences Cat#557741 | Mouse BALB/c IgG1, κ |
| CD45RA | Alexa Fluor 700 | HI100 | BD Biosciences Cat#560673 | Mouse IgG2b, κ |
| CCR7 | PE | 3D12 | BD Biosciences Cat#552176 | Rat IgG2a, κ |
| CD154 (CD40L) | FITC | TRAP1 | BD Biosciences Cat#558988 | Mouse BALB/c IgG1, κ |
| CD137 (4–1BB) | BV421 | 4B4–1 | BD Biosciences Cat#564091 | Mouse BALB/c IgG1, κ |
| CD69 | PE-Cy5 | FN50 | BD Biosciences Cat#555532 | Mouse IgG1, κ |
| CD134 (OX40) | APC-Cy7 | Ber-ACT35 | Biolegend Cat#350022 | Mouse IgG1, κ |
| CD95 (FAS) | APC | DX2 | BD Biosciences Cat#558814 | Mouse C3H/Bi IgG1, κ |
| Granzyme B | PE-CF594 | GB11 | BD Biosciences Cat#562462 | Mouse BALB/c IgG1, κ |
| IFN-γ | BV650 | 4S.B3 | BD Biosciences Cat#563416 | Mouse BALB/c IgG1, κ |
| IL-10 | BV786 | JES3–9D7 | BD Biosciences Cat# 564049 | Rat IgG1 |
| Ki67 | BV711 | B56 | BD Biosciences Cat#563755 | Mouse IgG1, κ |
| CD19 | Alexa Fluor 488 | HIB19 | BD Biosciences Cat# 557697 | Mouse IgG1, κ |
| CD20 | PerCP-Cy5.5 | 2H7 | BD Biosciences Cat#560736 | Mouse C57BL/6 IgG2b, κ |
| CD23 | Alexa Fluor 700 | M-L233 | BD Biosciences Cat#563673 | Mouse IgG1, κ |
| CD27 | APC-H7 | M-T271 | BD Biosciences Cat# 560222 | Mouse BALB/c IgG1, κ |
| CD86 | PE-CF594 | 2331(FUN-1) | BD Biosciences Cat#562390 | Mouse BALB/c IgG1, κ |
| CD80 | BV650 | 2D10.4 | BD Biosciences Cat# 751725 | Mouse IgG1, κ |
| CD40 | PE-Cy7 | 5C3 | BD Biosciences Cat# 561215 | Mouse IgG1, κ |
| CD43 | BV605 | 1G10 | BD Biosciences Cat#563378 | Mouse IgG1, κ |
| CD22 | PE-Cy5 | HIB22 | BD Biosciences Cat#555425 | Mouse IgG1, κ |
| CD24 | BUV395 | ML5 | BD Biosciences Cat#563818 | Mouse IgG2a, κ |
| CD38 | BUV737 | HB7 | BD Biosciences Cat#612824 | Mouse IgG1, κ |
| CD138 | BV711 | MI15 | BD Biosciences Cat#563184 | Mouse BALB/c IgG1, κ |
| CD5 | BV421 | UCHT20 | BD Biosciences Cat#562646 | Mouse BALB/c IgG1, κ |
| IgD | PE | IA6–2 | BD Biosciences Cat#555779 | Mouse BALB/c IgG2a, κ |
| IgM | APC | G20–127 | BD Biosciences Cat#551062 | Mouse IgG1, κ |
| IgG | BV786 | G18–145 | BD Biosciences Cat#564230 | Mouse IgG1, κ |
| Isotype | BV711 | X40 | BD Biosciences Cat#563044 | Mouse BALB/c IgG1, κ |
| Isotype | PE-Cy5 | MOPC-21 | BD Biosciences Cat#555750 | Mouse IgG1, κ |
| Isotype | APC | MOPC-21 | BD Biosciences Cat#555751 | Mouse IgG1, κ |
| Isotype | PE-CF594 | R3–34 | BD Biosciences Cat#562309 | Rat IgG1, κ |
| Isotype | BV421 | 27–35 | BD Biosciences Cat#562748 | Mouse C.SW IgG2b, κ |
| Isotype | BV605 | MOPC-21 | Biolegend Cat#400162 | Mouse IgG1, κ |
| Isotype | Alexa Fluor 488 | eBMG2b | Invitrogen Cat#53–4732-80 | Mouse / IgG2b, κ |
| Isotype | PE | MOPC-21 | BD Biosciences Cat#555749 | Mouse IgG1, κ |
| Isotype | PE-CF594 | X40 | BD Biosciences Cat#562292 | Mouse BALB/c IgG1, κ |
| Isotype | BV650 | X40 | BD Biosciences Cat#563231 | Mouse BALB/c IgG1, κ |
| Isotype | BV786 | R3–34 | BD Biosciences Cat#563847 | Rat IgG1, κ |