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. 2022 May 29;19(11):6629. doi: 10.3390/ijerph19116629

Figure 5.

Figure 5

Cl− influx and GABA-induced Cl− current-enhancing effects of PCET in superior cervical ganglion cells. (A) After 12 h of exposure to MQAE, Cl− selective fluorescence dye, superior cervical ganglion cells were filtrated at 380 nm in a FlexStation 3 reader and measured wavelengths emitted at 460 nm. Pentobarbital (10 μM) was used as a positive control, and the negative control was measured with no processing. PCET was administered 1 h before measurement, at a concentration range of 10–300 μg/mL. The data are shown as mean ± standard deviation. Experiments were repeated 10 times. Statistical significance was evaluated using the p-value, and the difference from the negative control was marked as ** p < 0.001. PCET increased the Cl− inflow into cells concentration-dependently. (B) The GABA-induced Cl− current strengthening effect was verified by treating 1 mg/mL and 3 mg/mL PCET on the Cl− current induced using a 10 s GABA (100 μM) treatment for a superior cervical ganglion cell fixed at −80 mV. The Cl− current increased according to the PCET treatment concentration.