FIG. 2.

Detection of NV RNA and internal control RNA by RT-PCR and oligoprobing of 10-fold (−1) and 100-fold (−2) dilutions of RNA extracts from ham samples seeded with decreasing concentrations of NV and processed by method 2. Lanes 9 and 10 are negative sample controls. Other lanes include an NV RT-PCR positive control (pos), a negative reagent control (neg), 50 copies of an internal standard control (int std), and a digoxigenin-labeled molecular size marker (MarkerVIII; Boehringer Mannheim) (marker). (A) Ethidium bromide-stained agarose gel; (B) Southern blot of gel in panel A. Numbers at the right are molecular sizes, in base pairs.