TABLE 1.
Detection of decreasing inputs of NV or HAV seeded onto foodstuffsa
| Foodstuff | No. of samples positive/no. tested at indicated input (PCRU)
|
|||||||
|---|---|---|---|---|---|---|---|---|
| 10,000
|
1,000
|
100
|
10
|
|||||
| NV | HAV | NV | HAV | NV | HAV | NV | HAV | |
| Ham | 2/2 | 2/2 | 2/2 | 2/2 | 3/4 | 2/2 | 0/3 | 1/2 |
| Turkey | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 1/2 | 2/2 |
| Roast beef | 2/2 | 3/3 | 2/2 | 2/2 | 3/3 | 1/3 | 2/3 | 0/2 |
a
Twenty to 30 g of food was seeded with decreasing inputs of both NV and HAV. Seeded samples were then processed by method 2, and 10- and 100-fold dilutions of extracted viral RNA were analyzed by RT-PCR and oligoprobing.