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. 2022 May 31;11(11):1811. doi: 10.3390/cells11111811

Figure 5.

Figure 5

DFO blocked HIV-1 Tat-, gp120-, and CQ-induced decreases in endolysosome numbers and increases in endolysosome volume. (A) Representative fluorescence microscopy images showing endolysosomes (red puncta) and nuclei (blue) and that DFO blocked CQ- (30 µM), Tat- (100 nM), and gp120- (1 nM) induced decreases in endolysosome numbers and increases in endolysosome volume. (B,C) Quantitatively, DFO blocked CQ-, Tat-, and gp120-induced decreases in endolysosome numbers and increases in endolysosome volume. Image analysis and quantification were performed from five independent experiments with 30 cells quantified for each experimental condition (n = 150). Error bars represent standard deviation (SD) of five independent experiments. A one-way ANOVA multiple comparisons test was used to compare control group and treatment group. Cells were chosen randomly during image acquisition and analysis from the microscope field of view, and no cells were intentionally excluded. Scale bars are 10 µm for the image and 3 µm for the inset. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.