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Effect of TJ356 stain exposed to four concentrations (12.5, 25, 50, and 100 µM) of ACR on subcellular DAF-16 nuclear localization (n ≥ 30 worms per group). Cytoplasm: The green fluorescence was diffusely distributed in the cytoplasm (A). Nuclear: The green fluorescence was concentrated in the nucleus and presented granularity (C–E). Intermediary: The green fluorescence was between the two states (B). ** compared with the control group, p < 0.01.
