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. 2022 May 26;19(11):6485. doi: 10.3390/ijerph19116485

Figure 11.

Figure 11

Effects of pretreatment with probiotics on the number of neutrophils, phase II. The groups selected in phase I (SAL30 and ACE 0.6% 30, now termed SAL, black bar and ACE, green bar) were compared with the corresponding two groups that were pretreated with 1 mL/day of Actimel® (ACT), 7 days before performance of the visceral sensitivity test (SAL + ACT black gridded bar, ACE + ACT green gridded bar). The number of neutrophils (A) was assessed using immunohistochemical methods in ten random fields per section. Data represent mean ± SEM. No statically significant differences were found. One-way ANOVA followed by Bonferroni post hoc test (n = 8–13). (B) Photomicrographs of myeloperoxidase immunopositive cells (neutrophils, see circles) in colonic mucosa after intracolonic administration of SAL (black frame) or in animals pretreated with Actimel® after intracolonic administration of saline (SAL + ACT; black dotted frame) or 0.6% acetic acid (ACE + ACT) green dotted frame). Scale bar = 50 μm.