TABLE 3.
Dehalogenating activity of crude extracts of M. avium MU1 and M. smegmatis CCM 4622 toward several halogenated aliphatic compounds
| Substrate | Product | Sp act (mU/mg of protein)a
|
|
|---|---|---|---|
| M. avium MU1 | M. smegmatis CCM 4622 | ||
| 1-Bromopropane | 1-Propanol | 0.87 | 3.58 |
| 1,3-Dibromopropane | 3-Bromo-1-propanol | 1.23 | 2.50 |
| 1-Bromo-2-methylpropane | NA | NA | |
| 1-Chlorobutane | 1-Butanol | 0.11 | 0.42 |
| 1-Bromobutane | 1-Butanol | 0.66 | 1.12 |
| 1-Chlorohexane | 1-Hexanol | 1.02 | 2.62 |
| 1-Bromohexane | 1-Hexanol | 0.77 | 0.91 |
| 1-Iodohexane | 1-Hexanol | 1.38 | 3.32 |
| 1-Chloroheptane | NA | NA | |
a
Micromoles of primary alcohol produced per minute per milligram of protein. NA,- not active. The detection limit of the gas chromatographic assay for primary alcohols was 0.007 to 0.015 mM.