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Optimization of conjugating conditions of QDs-N probe by using orthogonal tests.
| Group | The Ratio of EDC | The Ratio of N Protein | pH of PBS | FI of Test Line |
|---|---|---|---|---|
| 1 | 1:1500 | 1:2.5 | 7.4 | 1372 ± 24 |
| 2 | 1:2000 | 1:2.5 | 8.0 | 11,109 ± 22 |
| 3 | 1:2500 | 1:2.5 | 6.8 | 2368 ± 4 |
| 4 | 1:2500 | 1:5.0 | 7.4 | 3551 ± 10 |
| 5 | 1:1500 | 1:5.0 | 8.0 | 7375 ± 3 |
| 6 | 1:2000 | 1:5.0 | 6.8 | 6050 ± 4 |
| 7 | 1:2000 | 1:7.5 | 7.4 | 6145 ± 8 |
| 8 | 1:2500 | 1:7.5 | 8.0 | 24,797 ± 5 |
| 9 | 1:1500 | 1:7.5 | 6.8 | 16,817 ± 14 |
