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. 2022 Jun 6;23(11):6351. doi: 10.3390/ijms23116351

Figure 4.

Figure 4

Expression of neuropeptides in the ARC and PVN of standard diet (SD)- and high-fat diet (HFD)-fed cycling and ovx Npy1r2lox control and Npy1rrfb knockout female mice. Agouti-related protein immunoreactivity (AgRP-IR) in the ARC (A) and in the PVN (B) of SD- and HFD-fed cycling and ovx Npy1r2lox and Npy1rrfb females. HFD and ovx significantly decreased AgRP expression in the ARC of female mice. SD-fed cycling Npy1rrfb conditional mutants showed a significant decrease of AgRP-IR in the ARC compared to SD-fed cycling control females. Data are the mean ± SEM; n = 8–9 (ARC) and n = 7–10 (PVN) from 2 litters. ** p < 0.01 and ### p < 0.001 versus SD-fed cycling Npy1r2lox females; §§ p < 0.01 versus SD-fed cycling Npy1r2lox; mice; ## p < 0.01 versus SD-fed ovx Npy1r2lox female mice. (C) Representative images of the ARC of ovx females expressing AgRP-IR immunoreactivity (scale bar: 150 μm). Neuropeptide Y immunoreactivity (NPY-IR) in the ARC (D) and in the PVN (E) of SD- and HFD-fed cycling and ovx Npy1r2lox and Npy1rrfb females. A significant decrease of NPY-IR was observed in the ARC of ovx Npy1r2lox mice compared with cycling Npy1r2lox mice. A significant increase of NPY-IR was observed in the ARC of ovariectomized Npy1rrfb mice compared with their control littermates on the same diet regimen. § p < 0.05 versus cycling Npy1r2lox females on the same diet regimen. ** p < 0.01 versus ovx Npy1r2lox on the same diet regimen. Data are the mean ± SEM; n = 5–8 from 2 litters. (F) Representative images of the ARC of ovx females expressing NPY-IR immunoreactivity (scale bar: 150 μm). Proopiomelanocortin immunoreactivity (POMC-IR) in the ARC (G) and in the PVN (H) of SD- and HFD-fed cycling and ovx Npy1r2lox and Npy1rrfb females. A significant decrease of POMC-IR was observed in the ARC of SD-fed ovx Npy1rrfb mice compared with SD-fed ovx Npy1r2lox mice. Data are the mean ± SEM; n = 7–11 from 2 litters. *** p < 0.001 versus SD-fed ovx Npy1r2lox mice. (I) Representative images of the ARC of ovx females expressing POMC-IR (scale bar: 150 μm).