Table 1.
Statistical analysis.
| Measures | Female Mice | ANOVA | Variables | Interactions |
|---|---|---|---|---|
| Body weight | Cycling | 2-way RM |
Diet: F(1,101) = 80.54, p = 0.000 | Days–diet: F(11,1111) = 22.06, p = 0.000 |
| ovx | 2-way RM |
Genotype: F(1,96) = 13.99 p = 0.000 Diet: F(1,96) = 91.04, p = 0.000 |
Days–diet–genotype: F(11,1056) = 4.41; p = 0.000 Days–genotype: F(11,1056) = 7.35 p = 0.000 Days–diet: F(11,1056) = 107.36, p = 0.000 |
|
| Subcutaneous WAT | Cycling and ovx | 3-way | Genotype: F(1,94) = 12.71, p = 0.001 Diet: F(1,94) = 279.77; p = 0.000 ovx: F(1,94) = 283.34; p = 0.000 |
Genotype–ovx: F(1,94) = 5.47; p = 0.022 ovx–diet: F(1,94) = 17.43; p = 0.000 |
| Visceral WAT |
Cycling and ovx | 3-way | Diet: F(1,94) = 185.31; p = 0.000 ovx: F(1,94) = 116.32; p = 0.000 |
Genotype–diet: F(1,94) = 5.43; p = 0.022 |
| Gonadic WAT | cycling and ovx | 3-way | Diet: F(1,94) = 232.01; p = 0.000 ovx: F(1,94) = 33.14; p = 0.000 |
Diet–ovx: F(1,94) = 26.18; p = 0.000 |
| Leptin | Cycling and ovx | 3-way | Diet: F(1,117) = 107.80, p = 0.000 ovx: F(1,117) = 120.41, p = 0.000 |
|
| Spontaneous locomotor activity | Cycling and ovx | 3-way | Diet: F(1,82) = 16.58; p = 0.000 ovx: F(1,82) = 41.42; p = 0.000 |
Genotype–ovx–diet: F(1,82) = 5.19; p = 0.025 ovx–diet: F(1,82) = 7.7905; p = 0.007 |
| Cumulative energy intake | Cycling and ovx | 3-way | Diet: F(1,24) = 52.50, p = 0.000 | |
| 9-day energy intake | Cycling and ovx | 3-way RM |
Diet: F(1,240) = 52.50, p = 0.000 | |
| GTT | Cycling and ovx | 3-way RM |
Diet: F(1,112) = 25.62; p = 0.000 ovx: F(1,112) = 10.62; p = 0.002 |
Diet–hours: F(3,336) = 7.46; p = 0.000 |
| GTT AUC | Cycling and ovx | 3-way | Diet: F(1,112) = 26.38; p = 0.000 ovx: F(1,112) = 9.06; p = 0.003 |
|
| ITT | Cycling and ovx | 3-way RM |
Diet: F(1,80) = 9.74; p = 0.003 | Diet–hours: F(3,240) = 6.08; p = 0.001 |
| ITT AUC | Cycling and ovx | 3-way | Diet: F(1,80) = 10.21; p = 0.002 | |
| PTT | Cycling and ovx | 3-way RM |
Diet: F(1,85) = 19.04; p = 0.000 | Diet–ovx: F(1,85) = 5.12; p = 0.026 |
| PTT AUC | Cycling and ovx | 3-way | Diet: F(1,85) = 19.23; p = 0.000 | |
| Heart rate | Cycling and ovx |
3-way | ovx: F(1,80) = 15.39; p = 0.000 | |
| ARC AgRP-IR | Cycling and ovx | 3-way | Diet: F(1,62) = 31.34, p = 0.000 ovx: F(1,62) = 21.1, p = 0.000 |
Genotype–diet: F(1,62) = 7.80, p = 0.007 |
| PVN AgRP-IR | Cycling and ovx | 3-way | Genotype: F(1,57) = 4.54, p = 0.037 Diet: F(1,57) = 12.13, p = 0.001 ovx: F(1,57) = 14.24, p = 0.000 |
|
| ARC NPY-IR | Cycling and ovx | 3-way | Genotype: F(1,45) = 5.11, p = 0.029 ovx: F(1,45) = 4.82, p = 0.033 |
Genotype–ovariectomy: F(1,45) = 4.35, p = 0.043 ovx–diet: F(1,45) = 5.38, p = 0.025 |
| ARC NPY-IR | ovx | 2-way | Genotype: F(1,21) = 10.44, p = 0.004 Diet: F(1,21) = 6.11, p = 0.022 |
|
| ARC POMC-IR | Cycling and ovx | 3-way | Genotype: F(1,68) = 15.00; p = 0.000 | Genotype–ovx: F(1,68) = 6.86; p = 0.011 Diet–ovx: F(1,68) = 5.44; p = 0.023 |
| PVN POMC-IR | Cycling and ovx | 3-way | Diet: F(1,61) = 4.04; p = 0.049 ovx: F(1,61) = 10.48; p = 0.002 |
|
| Microglia cb/c | Cycling and ovx | 3-way | Genotype: F(1,32) = 5.95; p = 0.021 Diet: F(1,32) = 5.98; p = 0.020 ovx: F(1,32) = 36.01; p = 0.000 |
Genotype–ovx: F(1,32) = 6.35; p = 0.017 Diet–ovx: F(1,32) = 4.80; p = 0.036 |
| Microglia number | Cycling and ovx | 3-way | Genotype: F(1,32) = 5.91; p = 0.021 ovx: F(1,32) = 44.80; p = 0.000 |
Genotype–ovx: F(1,32) = 4.61; p = 0.039 Diet–ovx: F(1,32) = 4.27; p = 0.047 |
| Microglia cb/c | ovx | 2-way | Genotype: F(1,15) = 7.23, p = 0.017 Diet: F(1,15) = 6.32, p = 0.024 |
|
| Microglia number | ovx | 2-way | Genotype: F(1,15) = 8.80, p = 0.010 Diet: F(1,15) = 4.83, p = 0.044 |