TABLE 1.
Oligonucleotide primers used for PCR amplification and sequencing of human Ad and BKVa
| Virus type (region) | Position (nt) | Amplification reaction | Primer | Product size (bp) | Annealing temp (°C) | Sequence |
|---|---|---|---|---|---|---|
| Ad2 (hexon) | ||||||
| Ad40 (hexon) | 18858–18883 | First-left | HL | 301 | 55 | 5′-GCCGCAGTGGTCTTACATGCACATC-3′ |
| Ad41 (hexon) | 19136–19158 | First-right | HR | 5′-CAGCACGCCGCGGATGTCCAAAGT-3′ | ||
| Ad2 (hexon) | 18937–18960 | Nested-left | NHL | 143 | 55 | 5′-GCCACCGAGACGTACTTCAGCCTG-3′ |
| Ad2 (hexon) | 19051–19079 | Nested-right | NHR | 5′-TGTACGAGTACGCGGTATCCTCGCGGTC-3′ | ||
| BKV, JCV (VP2/VP3) | 1352–1367 | First-left | BJ1 | 765 | 46 | 5′-TATTGCMCCAGGAGGT-3′ |
| BKV, JCV (VP1) | 2132–2148 | First-right | BJ2 | 5′-AACATTTTCYCCTCCTG-3′ | ||
| BKV (VP1) | 1762–178 | Nested-left | BK4 | 290 | 50 | 5′-AGTAGATTTCCACAGGTTAG-3′ |
| BKV (VP1) | 1486–1506 | Nested-right | BK6 | 5′-CCAGGGGCAGCTCCCAAAAAG-3′ | ||
| BKV (VP1) | 2132–2148 | Seminested-left | BK2 | 388 | 46 | 5′-AACATTTTCCCCTCCTG-3′ |
| BKV (VP1) | 1758–1776 | Seminested-right | BK5 | 5′-GGACCTAACCTGTGGAAAT-3′ |
a
M = A + C; Y = C + T. For Ad, the sequence positions are referred to the Ad2 hexon region; for BKV and JCV, the sequence positions are referred to the BKV-Dunlop sequence.