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. 2022 Jun 5;23(11):6320. doi: 10.3390/ijms23116320

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) EV NTA analysis shows very similar profiles in terms of EV populations detected in all patient samples but reveals a very peculiar nanoparticle distribution for Pt# 61, which may deserve further investigation. (B) Expression of typical EV markers CD9, CD63, and CD81 evaluated by flow cytometry on a BD FACS Aria. (Upper panel) The expression of CD9 was almost absent in EVs consistently in all four patients; (middle panel) CD63 was detected in a comparable percentage in Pt#51, Pt#55, and Pt#60, while for Pt#61, it shows an almost double expression; (lower panel) EVs presented a similar expression of CD81 in Pt#51, Pt#55, and Pt#61 samples, while in Pt#60, it presented a lower expression. Some like 2.0E+5 mean 2.0 × 10⁵.