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. 2022 Jun 6;23(11):6335. doi: 10.3390/ijms23116335

Figure 2.

Figure 2

Sequencing of mtDNA revealed two mutations in Cika cattle. (A) Homoplasmic mutation T/C at mtDNA position 10,432 confirmed by Sanger sequencing. (B) The mutation (C allele) was found in two maternal lineages (Gavtraža and Pirha). The wild type (T allele) was present in all individuals not maternally related to Gavtraža and Pirha. Sequences of mutant and wild-type mtDNA around position 10432: (B1) Gavtraža’s sequence from the muscle tissue; (B2) Gavtraža’s sequence from the brain; (B3) Breza’s sequence; (B4) Košuta’s sequence; (B5) Cow 9502′s sequence; (B6) Cow 9505′s sequence; (B7) Holstein–Friesian control sample’s sequences; (B8–B12) Cika individuals not maternally related to Gavtraža and Pirha. All the sequences shown from B3 to B12 were obtained from blood cells. (C) NGS sequencing of the whole mitochondrial genome revealed an additional heteroplasmic mutation in Gavtraža (A/G) at position 11,081 bp, but not in Pirha.