Figure 1.

GCH1 expression is higher in melanoma cells. (A) Relative mRNA levels of GCH1 were determined in melanocytes, WM1552C, WM793, WM1366, Lu1205, and WM983B melanoma cells by real-time qPCR. (B,C) Protein expression of GTPCH1 in melanocytes and melanoma cells were determined by Western blot using a specific antibody. β-actin was used as the internal control. The Western blot image shows a representative result of three independent experiments. Values are reported in the bar graphs and expressed as the means ± S.D. The experiments were performed in triplicate, and p-values were based on the one-way ANOVA test, followed by Bonferroni’s post-test; *, p < 0.05; **, p < 0.01; and ***, p < 0.001.