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Tetrahydrobiopterin concentration is higher in metastatic melanoma cells. The amounts of BH4 (A); BH2 (B), BH4:BH2 ratio (C), and total biopterin (D) of melanocytes, WM1552C, WM793, and WM983C melanoma cells were determined by HPLC. Values are reported in the bar graphs and expressed as the means ± S.D. The experiments were performed in quintuplicate, and p-values were based on the one-way ANOVA test, followed by Bonferroni’s post-test; *, p < 0.05; **, p < 0.01; and ***, p < 0.001.
