Figure 4.

Melanoma cells exhibit increased protein expression of nitric oxide synthases. Expressions of eNOS (A), nNOS (B), and iNOS (C) in melanocytes and in WM1152C, WM793, and WM983B melanoma cells were determined by Western blot using specific antibodies. The Western blot images show representative results of three independent experiments. Values are reported in the bar graphs and expressed as the means ± S.D. The experiments were performed in quintuplicate, and p-values were based on the one-way ANOVA test, followed by Bonferroni’s post-test; *, p < 0.05 and ***, p < 0.001.