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. 2022 May 26;23(11):5979. doi: 10.3390/ijms23115979

Figure 5.

Figure 5

Tetrahydrobiopterin treatment restored the intracellular BH4 and recoupled NOS activity in radial growth melanoma cells. WM1552C cells were treated or not for 16 h with 20 and 40 μM BH4 and the amounts of BH4 (A), BH2 (B), BH4:BH2 ratio (C), and total biopterin (D) were determined by HPLC. The NO amount was evaluated by flow cytometry using DAF (E) and the O2•− levels using DHE (F). Values are reported in the bar graphs and expressed as the means ± S.D. The experiments were performed in triplicate, and the p-values were based on the one-way ANOVA test, followed by Bonferroni’s post-test. * p < 0.05, ** p < 0.01 and *** p < 0.001.