Skip to main content
. 2022 May 26;23(11):5979. doi: 10.3390/ijms23115979

Figure 6.

Figure 6

Tetrahydrobiopterin impaired the radial melanoma cell growth. WM1552C melanoma cells were treated (BH4) or not (NT) for 24, 48, 72 and 96 h with 10, 20 and 40 μM BH4, and viable cells were evaluated by MTT (A) for 9 days with 40 μM BH4, and the formation of clones was visualized by the clonogenicity assay (B,C) or for 5 days with 20 and 40 μM BH4, and the formation of tumorspheres was evaluated by the bead diameter measure (D,E). The viability of melanoma cells was also evaluated in the presence of 40 μM BH4 and 8.6 μM vemurafenib (F). Values are reported in the bar graphs and expressed as the means ± S.D. The experiments were performed in triplicate. p-values were based on the one-way ANOVA test, followed by Bonferroni’s post-test and two-way ANOVA test, followed by Bonferroni’s multiple comparisons test or by the Students’ t-test. *, p < 0.05; **, p < 0.01 and ***, p < 0.001.