Table 2.
Eumelanin decolorization with LMCS in different molar concentration ratios and at different reaction times (4 h, 24 h, and 48 h).
| Entry | LMCS | Molar Ratio 1 | DE%(4 h) 2 | DE%(24 h) 2 | DE%(48 h) 2 |
|---|---|---|---|---|---|
| 1 | ABTS/V | 0.5:1 | 15 | - | - |
| 2 | ABTS/V | 1:1 | 50 | 70 (76) 3 | 67 |
| 3 | ABTS/V | 2:1 | 18 | - | - |
| 4 | TEMPO/V | 0.5:1 | 22 | - | - |
| 5 | TEMPO/V | 1:1 | 46 | 63 (76) 3 | 66 |
| 6 | TEMPO/V | 2:1 | 26 | - | - |
| 7 | Syr/V | 0.5:1 | 24 | - | - |
| 8 | Syr/V | 1:1 | 55 | 67 (78) 3 | 67 |
| 9 | Syr/V | 2:1 | 28 | - | - |
| 10 | As/V | 0.5:1 | 19 | - | - |
| 11 | As/V | 1:1 | 39 | 51 (61) 3 | 69 |
| 12 | As/V | 2:1 | 12 | - | - |
| 13 | Av/V | 0.5:1 | 12 | - | - |
| 14 | Av/V | 1:1 | 31 | 46 | 47 |
| 15 | Av/V | 2:1 | 14 | - | - |
| 16 | Va/V | 0.5:1 | 28 | - | - |
| 17 | Va/V | 1:1 | 67 | 87 (96) 3 | 71 |
| 18 | Va/V | 2:1 | 32 | - | - |
| 19 | As/Av | 0.5:1 | 15 | - | - |
| 20 | As/Av | 1:1 | 34 | 38 | 38 |
| 21 | As/Av | 2:1 | 10 | - | - |
| 22 | Av/Syr | 0.5:1 | 2 | - | - |
| 23 | Av/Syr | 1:1 | 3 | 9 | 6 |
| 24 | Av/Syr | 2:1 | 1 | - | - |
| 25 | As/Syr | 0.5:1 | 8 | - | - |
| 26 | As/Syr | 1:1 | 16 | 28 | 38 |
| 27 | As/Syr | 2:1 | 11 | - | - |
1 Ratio applied in order to reach a total amount of 1.6 μmol of redox mediators. 2 Percentage of decolorization efficiency calculated after 4 h, 24 h, and 48 h of reaction. 3 DE% calculated after the second cycle of LMCS, expressed as total value of DE% obtained after the two treatments (referred to the initial mg of melanin).