TABLE 2.
Effect of temperature on enzyme activities
| Enzyme | Activity ata:
|
|||
|---|---|---|---|---|
| 37°C | 47°C | 58°C | 70°C | |
| 6-Phosphofructokinase | 0.11 (0.01) | — | — | — |
| 1-Phosphofructokinase | 0.07 (0.01) | — | — | — |
| PEPC | 0.16 (0.01) | 0.41 (0.03) | 0.77 (0.05) | 1.04 (0.13) |
| Malate dehydrogenase | 0.14 (0.06) | 0.28 (0.06) | 0.43 (0.08) | 0.65 (0.04) |
| Fumarase | 0.50 (0.04) | 0.69 (0.04) | 0.95 (0.11) | 1.56 (0.12) |
| Fumarate reductase | 0.06 (0.01) | 0.11 (0.01) | 0.15 (0.01) | 0.27 (0.02) |
| Pyruvate kinase | 0.17 (0.02) | 0.67 (0.02) | 1.20 (0.05) | 2.02 (0.29) |
| Lactate dehydrogenase | 0.30 (0.02) | 0.49 (0.03) | 1.05 (0.06) | 2.16 (0.13) |
| Pyruvate formate lysate | 0.21 (0.06) | — | — | — |
| Pyruvate ferredoxin oxidoreductase | 0.21 (0.02) | — | — | — |
| Hydrogenase | 0.06 (0.01) | — | — | — |
| Phosphotransacetylase | 0.97 (0.05) | 1.28 (0.04) | 1.61 (0.10) | 2.54 (0.29) |
| Acetaldehyde dehydrogenase | 0.07 (0.00) | — | — | — |
| Alcohol dehydrogenase | 0.13 (0.01) | 0.26 (0.02) | 0.42 (0.03) | 0.43 (0.03) |
| Acetate kinase | 0.29 (0.01) | 0.48 (0.02) | 0.59 (0.04) | ND |
a
In micromoles per minute per milligram of protein. ND, not detected; —, not determined. The standard errors of the measurements are in parentheses.