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. 2009 Nov 26;13(8b):2547–2558. doi: 10.1111/j.1582-4934.2008.00657.x

Figure 2.

Figure 2

A and B, RT‐PCR analysis of human MSC mRNA, at passage 4, using TaqMan® probes. Pro‐inflammatory molecules tested: IL2, IL4, IL5, IL8, IL12p40, INFγ and TNFα (A); Anti‐inflammatory molecules tested: IL6, IL10, IL13 and TGFβ1 (B). C and D, RT‐PCR analysis of rat MSCs at passage 4 with primers. Pro‐inflammatory molecules tested: IL2, IL4, IL12p35 & p40, IL16, IL17, INFγ (C); Anti‐inflammatory molecules tested: IL6, IL10, IL13 and TGFβ1 (D). E. Gene expression of cytokines in human MSCs. Gene expression absolute quantifications were realized by qPCR for anti‐inflammatory cytokines (IL6, TGFβ1) and pro‐inflammatory cytokines (IL8, IL12) with standards curves (data not shown). Experiments were performed on 3 different samples in duplicates. Human MSCs are characterized by 2717 and 1707 IL6 and TGFβ1 transcripts per ng of cDNA respectively as compared to 54.80 and 42.80 IL8 and IL12 transcripts per ng of cDNA, respectively. There are about a 50‐fold and a 30‐fold more IL6 and TGFβ1 transcripts than IL8 and IL12 transcripts, respectively. F. Supernatants were collected from human MSCs propagated for 4 passages and analysed by ELISA to determine levels of IL‐6, IL12p40 and TGFβ1. Data presented are representative of 2 independent experiments. No pro‐inflammatory IL12p40 presence was detected as compared to the 2 anti‐inflammatory cytokines TGFβ1 and IL6.