Figure 4.

Strontium ranelate (SrRan) activates Akt signalling in osteoblasts. Osteoblasts from [CaSR^+/+] and [CaSR^−/−] mice were cultured in DMEM supplemented with 5% FCS for 24 hrs, then treated with or without increasing doses of SrRan at the indicated concentrations for 10 (A, B) or 30 min. (C, D). Phosphorylation level of Akt was evaluated by Western blot analysis, relative quantification was performed after correction for β‐actin content, and the results expressed as treated over control ratio (T/C).