TABLE 2.
Properties of purified 2,6-β-d-fructan 6-levanbiohydrolase from S. exfoliatus F3-2 and of the other levanbiose-producing LDEs
| Microorganism | Mol wt
|
Optimum condition
|
Stability range
|
pI | Mode of action on levan | Effect on β-2, 1- linkages of fructose | Products from levana | Reference | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| SDS-PAGE | Gel filtration | pH | Temp (°C) | pH | Temp (°C) | ||||||
| Arthrobacter sp. strain 51A | 60,000 | 64,000 | 5.8 | 65 | 7.0–9.0 | ≦45 | 6.4 | Endo | Yes | F, F2 | 8, 9 |
| Streptomyces sp. strain 7-3 | 54,000 | 57,000 | 6.5 | 40 | 5.5–8.5 | ≦40 | 4.7 | Exo | No | F, F2, F3 | 9, 10 |
| S. exfoliatus F3-2 | 54,000 | 60,000 | 5.5 | 60 | 3.5–8.0 | ≦50 | 4.7 | Exo | Yes | F, F2 | This study |
a
F, frucose; F2, levanbiose; F3, levantriose.