Figure 2.

MIM-4 displays slight anti-proliferative effects on colon carcinoma cells cultivated in 2D, under starvation. (A) Representative scheme of the experimental protocol. HCT-116 cells were serum-starved for 24 h, and then treated with either MIM-4 or Veh. for a period of 72 h. The medium and the treatments were freshly renewed every day during the course of the treatment. The cells were kept in a starved state, by being supplemented or not with only 1% FBS. AA: amino acids. (B) Pictures were taken every day and representative pictures, taken with Evos XL Transmitted light microscope at the x 20 magnification, are presented. (C) Viable cells were manually counted by the trypan blue exclusion method at the end of the treatment. This experiment was performed once, and the results are presented as the mean ± SD of viable cell counts, repeated three times, as technical replicates. The black dot line was drawn in the graph to better visualize the effect of MIM-4 compared to its Veh. control. (D) The graph shows the results of the two experiments performed as the percentage of viable cells normalized on the respective Veh. (set to 100%). Each black dot represents the normalized results for one tested MIM-4 capsule.