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. 2022 Jun 2;23(11):6239. doi: 10.3390/ijms23116239

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunoblotting detection and quantitative analysis for pERK and ERK 1/2. (A) Total lysates of control and V-treated (1 mM, 500 μM) embryos after 24, 30, 36 and 42 h of development/treatment. Actin was used as a loading control. Histograms show the densitometric analysis of bands identified for (B) pERK and (C) ERK 1/2. Relative protein expression, reported as arbitrary units, was calculated as the band density ratio to that of actin. Experiments were performed in triplicate and data are expressed as means ± standard deviation (n = 3 ± SD). Data were analyzed by one-way ANOVA. Treatments with the same lowercase letter do not differ (Tukey HSD).