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. 2022 Jun 2;23(11):6239. doi: 10.3390/ijms23116239

Figure 5.

Figure 5

Fluorescent TUNEL assay and densitometric analysis. Pictures of demonstrative embryos at 24, 30, 36 and 42 h of development/treatment. DNA fragmentation (A1–N1). Nuclei marked with propidium iodide (A2–N2). Merge of both signals (A3–N3). Control embryos (A1–A3,D1–D3,H1–H3,K1–K3); 1 mM V-treated embryos (B1–B3,E1–E3,I1–I3,L1–L3); 500 μM V-treated embryos (C1–C3,F1–F3,J1–J3,M1–M3). Positive control embryo at 42 h of development (G1–G3). Negative control embryo at 42 h of development (N1–N3). Bar = 100 μm. Histograms showing data related to the quantitative analysis of fluorescence from apoptotic DNA. Experiments were performed in triplicate and data are expressed as means ± standard deviation (n = 3 ± SD). Data were analyzed by one-way ANOVA. Treatments with the same lowercase letter do not differ (Tukey HSD).