TABLE 1.
t (h) | Sp act and absorbance in:
|
|||||
---|---|---|---|---|---|---|
Nonaerated culture
|
Aerated culture
|
|||||
A600 | NADH oxidase | LDH | A600 | NADH oxidase | LDH | |
2 | 0.2 | 0.56 | 9.16 | 0.2 | 0.56 | 9.16 |
2.5 | 0.42 | 0.69 | 8.31 | 0.40 | 0.57 | 9.94 |
3.5 | 0.86 | 0.52 | 9.28 | 0.62 | 0.55 | 8.09 |
4.5 | 1.04 | 0.67 | 12.2 | 0.67 | 0.51 | 7.17 |
6 | 1.2 | 0.61 | 12 | 0.68 | 0.75 | 9.28 |
Values represent specific activity in micromoles of NADH reoxidized per minute per milligram of protein during the growth of L. delbrueckii subsp. bulgaricus in MRS medium with 2% lactose at 42°C in aerated and nonaerated cultures.