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. 2022 Jun 9;8(6):e09660. doi: 10.1016/j.heliyon.2022.e09660

Figure 5.

Figure 5

TBrC and L-theanine (T) suppressed the growth of human lung cancer A549 and H460 cells as well as host nuclear transcriptional activation of NF-κB p65 in A549 cells without affecting the cell viability of normal human embryonic lung fibroblasts (MRC-5 cells). (A–C) TBrC and T displayed significant growth inhibition of lung cancer A549 (A) and H460 (B) cell lines without affecting the cell viability of normal human lung MRC-5 cells (C) at the indicated concentrations (48 h treatment), but Bay11-7082 (Bay/0.62 μg/mL, a specific inhibitor of NF-κB) significantly suppressed the normal MRC-5 cell growth. (D) The TNFα (20 ng/mL) induced the nuclear transcriptional activation of NF-κB p65 in human lung cancer A549 epithelial cells was effectively inhibited by TBrC (50 μg/mL), T (50 μg/mL) and Bay (0.62 μg/mL), determining by the Trans AM NF-κBp65 Transcription Factor Assay Kit. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared to control group; $$$p < 0.001, compared to TNFα group; ###p < 0.001, compared to L-theanine (T) group or TNFα + L-theanine (T) group.