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Amplification product of 5.6 kb of the ccpA region of L. pentosus on a 1% agarose gel. Sizes of DNA fragments are indicated in kilobase pairs. Lane 1, DNA marker fragments; lane 2, 3 μl of a 50-μl-volume inverse PCR using oligonucleotides LPE1 and LPE2 and religated L. pentosus MD363 chromosomal DNA.
